How to Make a Wet Mount Slide

How to Make a Wet Mount Slide

A wet mount slide is the most typical kind of slide preparation for microscope work. If you deal with microscopic lens or are planning to start, you need to learn how to make a wet mount microscopic lense slide.

Preparing a Wet Mount Slides

Prior to you start building your slides, make sure you have whatever you will need, including slides, cover slips, droppers or pipets and any chemicals or stains you plan to use.

You will be utilizing 2 primary types of slides, 1) the typical flat glass slide, and 2) the anxiety or well slides. Well slides have a small well, or imprint, in the center to hold a drop of water or liquid compound. They are more pricey and generally used without a cover slip.

Standard slides can be either plastic or glass and are 1 x 3 inches (25 x 75 mm) in size and 1 to 1.2 mm thick.

Wet slides will use a cover slip or cover glass, a very thin square piece of glass (or plastic) that is positioned over the sample drop. Without the cover in place, surface area stress would trigger the bead to bunch up in a dome. The cover breaks this tension, flattening the sample and permitting very close inspection with very little focusing. The cover also serves to protect the objective lens from interfering with the sample drop.

How to Make a Wet Mount Microscope Slide

How to Make a Wet Mount Slide
  1. Use a flat glass slide to prepare a wet mount. The slide should be clean and devoid of dust or other fine particles.
  2. Suck up a couple of drops of your liquid specimen into a medicine dropper.
  3. Pick up a flat glass slide with one hand, grasping it by the outer edges.
  4. Location one drop of the liquid specimen in the medication dropper on top of the slide. Make certain the drop goes on the center portion of the slide.
  5. Put down the medication dropper. Do not tilt the slide in your other hand while you are doing this.
  6. Use your liberty to carefully get a cover slip (they are very fragile). Understand the cover slip by the outer edges.
  7. Place the cover slip on top of the slide, making certain the edges of the cover slip match up with the edges of the slide. Do not push down on the cover slip.
  8. Continue to grasp the slide/cover slip combination by the outer edges. You ought to keep the slide as horizontal and consistent as possible, and place it on the viewing tray of your microscopic lense.

What is a Wet Mount Slide Used for?

Used for aquatic samples, living organisms and natural observations, wet mounts suspend specimens in fluids such as water, salt water, glycerin and immersion oil.

Wet mount slides are used to view living organisms, along with liquid substances of all kinds. They are also used for any sort of specimen that needs to be kept wet.

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Morris T. Parson, Tech Support

Leading specialist in electronics and engineering. Author of dozens of articles on various topics from the world of hi-tech. Sound independent analysis of products, exploring new niches and global trends is his special passion.

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Did you make a wet mount slide before?

  1. Jane

    We used following steps in our university in Kentucky to prepare wet mount slides:

    – Using a pipette, place a drop of water on the specimen.
    – Then place on edge of the cover slip over the sample and thoroughly lower the cover slip into place utilizing a toothpick or equivalent.

    This approach will assist prevent air bubbles from being caught under the cover slip.